A gas-chromatographic method for separating and measuring barbiturates and glutethimide in blood.
نویسندگان
چکیده
A gas-chromatographic method is described for the quantitative deter. mination of 11 barbiturates and glutethimide in blood. The extracted barbiturates are methylated and injected into a 7% DC-200 column. Use of methohexital as the internal standard, as well as the addition of standards to a drug-free sample of blood, compensates for losses and for differences in the distribution coefficient of the individual barbiturates, and makes the procedure highly precise and accurate.
منابع مشابه
Gas-liquid chromatographic isolation, identification, and quantitation of some barbiturates, glutethimide, and diphenylhydantoin in whole blood.
An accurate method is described for the qualitative and quantitative determination of 11 barbiturates, glutethimide, and diphenylhydantoin in a single extract obtained from 1 ml of whole blood, which is extracted with ether at pH 2 and separately but simultaneously gas-liquid chromatographed on a selective (3% OV-17) and a nonselective (5% OV.1) column. Retention data are given. Results for bar...
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T HE POSSIBILITY OF DRUG intoxication frequently arises in patients admitted to the hospital in coma. Ingestion of salicylates by children and of barbiturates by adults are the most common causes. Since the introduction of glutethimide (2-ethyl-2-phenylglutarimide) in 1954, there have been increasing reports implicating this drug in drug intoxication. Since the clinical picture of glutethimide ...
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عنوان ژورنال:
- Clinical chemistry
دوره 17 10 شماره
صفحات -
تاریخ انتشار 1971